This record contains data collected on the RV Southern Surveyor, in the Indian ocean during September 2012. Samples were collected between Fremantle and Darwin. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, and the absorption coefficient for particulate (a/p) and detrital or non-algal (a/d) components of the water column. A total of 9 stations were sampled.